Objective To research the serial toxic effects of 0.05% fipronil cockroach gel bait on adults and nymphs of Blattella germanica. Methods This experiment used pipette to offer insecticide in the first efficacy experiment; and the serial toxic effect experiment was conducted using the dead insect of last serial toxic effect experiment to feed the cockroach. Results The first to fourth secondary effects of tested insecticide on adults reached 100%, the fifth secondary effects from the remains amounted to 78.3%. Against nymphs, however, only the first two secondary effects reached 100%, the third and fourth mortality were only 48.3% and 15.0%. The results indicated that 0.05% fipronil cockroach gel bait can continuously controlB. germanica effectively and it was more effectively on adults than on nymphs. Conclusion Fipronil has a good serial toxic effect on B. germanica.

Objective To identify the eclosion time of Culex pipiens pallens and Aedes albopictus for best getting the experimental females. Methods Rearing Cx. pipiens pallens and Ae. albopictus with standard rearing technique and study on numbers of females and males at different time of eclosion. Results The peak of adult emergence occured at 2nd and 3rd day after eclosion and most adults emergenced within 4 days. Adults was predominantly male about 2 d prior to adult eclosion. Two days later, females were the main part of emergenced adults. After all adults emergenced, gender ratio was close to 1:1. Conclusion Taking adults emergencing at 3-4 day will obtain high ratio of females which as materials for pesticide registration could meet the needs of experiment.

Objective To investigate the resistance dynamics of Musca domestica in the 26 years in Jinan city for the development of its control strategies. Methods The micro-drop method was used for bioassay of M. domestica collected in different years in different parts of the city to determine its resistance status and trends. Insecticides dissolved in acetone were applied topically on the thoracic notum of female adults. Results M. domestica in Jinan city and surrounding area in 1982 were susceptible to conventional pyrethrin chemicals. However, 26 years later, the susceptibility of M. domestica to conventional pyrethrin chemicals decreased to varying degrees. Compared with the susceptible strain, the resistance of field housefly to deltamethrn and to bata-cypermethrinwas was 75.00-211.67 and 25.52-116.21 folds in 2008 respectively, indicating that the degree of housefly resistance was at a medium or high level. Conclusion The resistance of M. domestica to pyrethrin chemicals in Jinan and the surrounding areas has developed to varying degrees. Strategies for the control of resistance should be developed based on the local conditions.

Objective To determine the efficacy and feasibility of synthetic amorphous silica against Dermanyssus gallinae. Methods Forced contact tests were used in accordance with GB/T 13917.1-2009. Results The 3% synthetic amorphous silica was painted on three types of board at 6 g a.i/m². For cement boards, the 24 h mortality was 100% and the residual efficacy lasted about 45 days; for silicate glass cotton board, the 24 h mortality was 100% and the residual efficacy lasted about 90 days; and for alkyd resin varnish boards, the 48 h mortality was 100% and the residual efficacy lasted about 60 days. Conclusion Synthetic amorphous silica was effective in the control of D. gallinae.

Objective To develop standard laboratory rearing techniques for Aedes albopictus in order to provide consistent, quality, standardized testing insects for use in pesticide experiments. Methods Using standard rearing techniques, the mosquitoes were bred under controlled rearing conditions. The reliability of multiregional rearing was examined based on the weight of female adults 3 days after eclosion from three batches of mosquitoes. Results All three batches resulting from standard rearing techniques yielded qualified Ae. albopictus based on the average weight, with high repeatability and low relative deviation (8.28%) of weights. As shown from the average weights of mosquitoes bred using different dietary formulas, all three approaches were applicable to different regions and the relative deviation was low (7.69%). Conclusion The rearing technique was repeatable and effective in developing weight-uniform Ae. albopictus for pesticide experiments.

Objective To explore the test methods for ant bites and to determine the killing effect of imidacloprid baits against Monomorrium pharaonis. Methods Laboratory test, simulated field test and field test were carried out. Results The LT50 value of 0.03% imidacloprid baits for M. pharaonis was 21.10 h, with a 7 d mortality of 100%. In the simulated field test, all of 4 queen ants were killed at day 5, while 100% of ergates died at day 6. In the field test, the density of M. pharaonis reduced to 98.42% at the 14 day. Conclusion Using M. pharaonis as the subject, the laboratory and simulated field test were applicable for ant baits, and 0.03% imidacloprid baits had favorable killing effect against M. pharaonis, which could be used for residential termite control.

Objective To study the evaluation methods of larvicides registration and field test.Methods Larvae were collected from breeding sites in the external environment or adult mosquitoes were trapped to oviposit in man-made areas.Results The relative population index(RPI)of larvae from breeding sites was under 5 after treatment by 1%temephos granules for 4 weeks,which had a good control effect.RPI of larvae from wasted tyres was also less than 5 after treatment for 4 weeks,while it was under 10 after treatment for 3 weeks in cement pool.Conclusion The field control effect and registration of quick-acting insecticides could be evaluated by the above mentioned two methods.

【Abstract】 Objective To study the evaluation methods of larvicides registration and field test. Methods Larvae were collected from breeding sites in the external environment or adult mosquitoes were trapped to oviposit in man-made areas. Results  The relative population index (RPI) of larvae from breeding sites was under 5 after treatment by 1% temephos granules for 4 weeks, which had a good control effect. RPI of larvae from wasted tyres was also less than 5 after treatment for 4 weeks, while it was under 10 after treatment for 3 weeks in cement pool. Conclusion The field control effect and registration of quick-acting insecticides could be evaluated by the above mentioned two methods.